Nov 10, 20232 min readWestern Blot – 5. BlockingBlocking prevents antibodies from binding to the membrane nonspecifically minimizing background signals. Two different blocking solutions...
Nov 8, 20233 min readWestern Blot – 4. StainingYou can skip staining step. However, if you want to know that proteins were transferred from the gel to membrane, you can stain either...
Jul 10, 20231 min read260/280 and 260/230 RatiosWhat is NanoDrop? NanoDrop spectrophotometer is used to measure DNA, RNA or protein concentration and its purity. It can be used to...