Blocking prevents antibodies from binding to the membrane nonspecifically minimizing background signals. Two different blocking solutions...

You can skip staining step. However, if you want to know that proteins were transferred from the gel to membrane, you can stain either...

In this step, proteins will be moved from the gel to the membrane. There are three main methods for transferring: tank transfer systems,...

Gel Preparation (XCell SureLock Mini-Cell Electrophoresis System, Invitrogen) Separating Gel Prepare separate gel solution in 50 ml tube...

Western blot is used to detect proteins in the sample. In this technique, proteins are separated by their molecular weight during gel...

Now, you have agarose beads with the target proteins. Depending on your purpose, you can process IP, co-IP or RNA-IP (RIP). For...

Input When I’ve just started learning IP in grad school, the concept of input was hard to understand. So, I wanted to talk about input in...